Procedure of Plant tissue culture
The part(s) of the plant used for culturing is known as explants. The explants are cultured in-vitro on a nutrient medium that caters to fulfil its nutritional requirements. The nutrient medium must provide the following:-
- Macronutrients – This includes elements like nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S) which is required for proper growth and morphogenesis.
- Micronutrients – Elements like iron (Fe), manganese (Mn), zinc (Zn) etc., which are also crucial to the growth of tissues.
- Carbon or Energy source – This is one of the most crucial ingredients of the nutrient media. Sucrose is the most widely used carbon source among other carbohydrates that serve to provide C, H, and O.
- Vitamins, amino acids, and other inorganic salts.
Apart from these, the culture media also serves as a medium for supplying phytohormones or plant growth regulators to the issues which bring about their morphogenesis as per requirement. The tissues of the explants first lose their specificity to form a hard brown lump known as callus. The callus then splits to develop a plant organ or a whole new plant depending upon the quantity and composition of phytohormones supplied. The entire process requires strict aseptic conditions to be maintained at all times as a single contamination can ruin an entire batch of plants.
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