Steps of Tissue Culture
The steps of tissue culture are given below:
Initiation Phase
At this stage, the tissue is initiated into the culture. The tissue of interest is obtained, introduced and sterilized to prevent the process from any contamination.
Multiplication Phase
In this stage, the sterilized explant is introduced into the medium composed of growth regulators and appropriate nutrients. They are responsible for the multiplication of cells. This undifferentiated mass of cells is known as a callus.
Root Formation
The roots start forming. Plant growth hormones are added to initiate the root formation. Consequently, we obtain a complete plantlets.
Shoot Formation
The plant growth hormones for the formation of shoot are added and the growth is observed for a week.
Acclimatization
When the plant starts developing, it is transferred to the greenhouse to develop under controlled environmental conditions. It is finally transferred to the nurseries to grow under natural environmental conditions.
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